10X Genomics Spatial Gene Expression samples are now being processed on the Visium CytAssist located at the Gene Expression Center. TRIP will be assisting with the first steps of the Visium with CytAssist protocol: Histology, RNA extraction from FFPE blocks used for QC, helping to find a pathologist for ROI selection (if needed) and Visium H&E image scanning.
For Visium questions and to schedule a meeting to discuss your project and timeline, contact Gene Expression Center at: gecinfo@biotech.wisc.edu
After your meeting with GEC, please submit a TRIP iLab request.
For information on TRIP’s portion of the Visium with CytAssist collaboration with GEC.
Poster Presentations-Proffered Abstract:
Stangis, M.: Cheng, L.; Albrecht, D.; Snow, S.; Wirtz, T.; Matkowskyj, K; Dinh, D,; Kendziorski, C.; Halberg, R. Abstract 6930: Spatial analysis of multi-ancestral tumors to understand how intercellular signaling impacts tumor invasion. [abstract] In: Proceedings of the American Association for Cancer Research Annual Meeting 2024: Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl): Abstract nr 6930.
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Information to provide TRIP for a Visium CytAssist project
Click here for detailed information.
Sample Size
Each slide being run on the CytAssist will have one tissue sample centered on the slide. Based on the size of the tissue, one of two gaskets will be used. A 6.5mm x 6.5mm gasket or an 11mm x 11mm gasket.