Karla Esbona

Credentials: Ph.D.

Position title: TRIP Scientific Manager/ Senior Scientist

Email: kesbona@wisc.edu


Dr. Esbona is the TRIP Laboratory Scientific Manager.  Her research focus is in cancer inflammatory and tumor microenvironment biomarkers.   She has interest in the automated quantitation of standardized histological stains as well as biomarker development. She has expertise with various multiplex IHC/IF/ISH/FISH techniques, spatial transcriptomics and computational imaging analysis.


Papadas A, Deb G, Cicala A, Officer A, Hope C, Pagenkopf A, Flietner E, Morrow ZT, Emmerich P, Wiesner J, Arauz G, Bansal V, Esbona K, Capitini CM, Matkowskyj KA, Deming DA, Politi K, Abrams SI, Harismendy O, Asimakopoulos F. Stromal remodeling regulates dendritic cell abundance and activity in the tumor microenvironment. Cell Rep. 2022 Aug 16;40(7):111201. doi: 10.1016/j.celrep.2022.111201. PMID: 35977482

Scribano CM, Wan J, Esbona K, Tucker JB, Lasek A, Zhou AS, Zasadil LM, Molini R, Fitzgerald J, Lager AM, Laffin JJ, Correia-Staudt K, Wisinski KB, Tevaarwerk AJ, O’Regan R, McGregor SM, Fowler AM, Chappell RJ, Bugni TS, Burkard ME, Weaver BA. Chromosomal instability sensitizes patient breast tumors to multipolar divisions induced by paclitaxel. Sci Transl Med. 2021 Sep 8;13(610):eabd4811. doi: 10.1126/scitranslmed.abd4811. Epub 2021 Sep 8. PMID: 34516829

Magee K, Marsh IR, Turek MM, Grudzinski J, Aluicio-Sarduy E, Engle JW, Kurzman ID, Zuleger CL, Oseid EA, Jaskowiak C, Albertini MR, Esbona K, Bednarz B, Sondel PM, Weichert JP, Morris ZS, Hernandez R, Vail DM. Safety and feasibility of an in situ vaccination and immunomodulatory targeted radionuclide combination immuno-radiotherapy approach in a comparative (companion dog) setting. PLoS One. 2021 Aug 12;16(8):e0255798. doi: 10.1371/journal.pone.0255798. eCollection 2021. PMID: 34383787

Jun Wan, Samuel Block, Rebecca Thiry, Karla Esbona, Anjon Audhya and Beth A. Weaver.  Mad1 destabilizes p53 by preventing PML from sequestering MDM2. Nature Communications. Submitted October 2018.

Sharick J, Jeffery J, Karim M, Walsh C, Esbona K, Cook R, Skala M. Cellular Metabolic Heterogeneity In Vivo Is Recapitulated in Tumor Organoids. Neoplasia. 2019, Vol: 21, Issue: 6, Page: 615-626. PMID: 31078067.

Sharick JT, Walsh CM, Sprackling CM, Pasch CA, Pham DL, Esbona K, Choudhary A, Garcia-Valera R, Burkard ME, McGregor SM, Matkowskyj KA, Parikh AA, Meszoely IM, Kelley MC, Tsai S, Deming DA, Skala MC. Metabolic Heterogeneity in Patient Tumor-Derived Organoids by Primary Site and Drug Treatment. Front Oncol. 2020 May 15;10:553. doi: 10.3389/fonc.2020.00553. eCollection 2020. PMID: 32500020

Huang W, Nebiolo C, Esbona K, Hu R, Lloyd R. Ki67 index and mitotic count: Correlation and variables affecting the accuracy of the quantification in endocrine/neuroendocrine tumors. Ann Diagn Pathol. 2020 Oct;48:151586. doi: 10.1016/j.anndiagpath.2020.151586. Epub 2020 Aug 15. PMID: 32836178

Singh A, Denu RA, Wolfe SK, Sperger JM, Schehr J, Witkowsky T, Esbona K, Chappell RJ, Weaver BA, Burkard ME, Lang JM. Centrosome amplification is a frequent event in circulating tumor cells from subjects with metastatic breast cancer. Mol Oncol. 2020 Aug;14(8):1898-1909. doi: 10.1002/1878-0261.12687. Epub 2020 May 19. PMID: 32255253

Wang Y, Hardin H, Chu YH, Esbona K, Zhang R, Lloyd RV. Long Non-coding RNA Expression in Anaplastic Thyroid Carcinomas. Endocr Pathol. 2019 Dec;30(4):262-269. doi: 10.1007/s12022-019-09589-y. PMID: 31468286

Esbona K, Yi Y, Saha S, Yu M, Van Doorn RR, Conklin MW, Graham DS, Wisinski KB, Ponik SM, Eliceiri KW, Wilke LG, Keely PJ. The Presence of Cyclooxygenase 2, Tumor-Associated Macrophages, and Collagen Alignment as Prognostic Markers for Invasive Breast Carcinoma Patients.  Am J Pathol. 2018 Mar;188(3):559-573. doi: 10.1016/j.ajpath.2017.10.025.  PMID: 29429545


Connor JP, Esbona K, Matkowskyj KA.  AKR1B10 expression by immunohistochemistry in surgical resections and fine needle aspiration cytology material in patients with cystic pancreatic lesions; potential for improved nonoperative diagnosis.  Hum Pathol. 2017 Dec;70:77-83. doi: 10.1016/j.humpath.2017.10.006. Epub 2017 Oct 24. PMID: 29079172


Esbona K, Li Z and Wilke L.  Intraoperative Imprint Cytology and Frozen Section Pathology for Margin Assessment in Breast Conservation Surgery: A Systematic Review. Ann Surg Oncol. 2012. PMID: 22847119

Esbona K, Inman D, Saha S, Jeffery J, Schedin P, Wilke L, Keely P.  COX-2 modulates mammary tumor progression in response to collagen density.  Breast Cancer Res. 2016 Mar 22;18(1):35. doi: 10.1186/s13058-016-0695-3.  PMID: 27000374