Anupama Singh

Biography

Anupama comes with over 15 years of research experience in the fields of Cancer and Immunology. Prior to joining the TRIP lab in the summer of 2022, she worked as Client Based Researcher at the Circulating Biomarker Core. Anu is particularly trained in immune cell functioning, circulating biomarkers, genetic and post-translational signaling and cell culturing. Anu, at TRIP lab enjoys exploring vast array of immuno-staining possibilities with the Ventana system and loves the challenge of optimizing multiplexed IHC protocols using  automated immuno-staining procedures.

Publications

Purohit TA, Gawdzik J, Armstrong EA, Yang B, Schultz Z, Bahr K, Do TJ, Machhi R, Sardeson S, Rizvi M, Nakada SA., Singh A, Esbona K, Huang W, Roskes ML, Khurana E, Lewis PW, Denu JM, Jarrard DF. Genetic loss of CHD1 regulates distinct histone post-translational modifications in the development of castration-resistant prostate cancer. Neoplasia March 01,2026 volume 73, article 1012289 2026.

Qinyuan Li, Singh A, Hu R, Huang W, Shapiro D, Abel EJ, Zong Y. GPR143, A novel immunohistochemical marker for renal tumors with FLCN/TSC/MTOR-TFE alterations. 2026. medRxiv paper print. https://doi.org/10.64898/2026.04.06.26350070;

Filon M, Yang B, Purohit TA, Schehr J, Singh A, Bigarella M, Lewis P, Denu J, Lang J, Jarrard DF. Development of a multiplex assay to assess activated p300/CBP in circulating prostate tumor cells. Oncotarget. 2023 Jul 20; 14:738-746. doi: 10.18632/oncotarget.28477. PMID: 37477521; PMCID: PMC10360924.

Zhao SG, Sperger JM, Schehr JL, McKay RR, Emamekhoo H, Singh A, Schultz ZD, Bade RM, Stahlfeld CN, Gilsdorf CS, Hernandez CI, Wolfe SK, Mayberry RD, Krause HM, Bootsma M, Helzer KT, Rydzewski N, Bakhtiar H, Shi Y, Blitzer G, Kyriakopoulos CE, Kosoff D, Wei XX, Floberg J, Sethakorn N, Sharifi M, Harari PM, Huang W, Beltran H, Choueiri TK, Scher HI, Rathkopf DE, Halabi S, Armstrong AJ, Beebe DJ, Yu M, Sundling KE, Taplin ME, Lang JM. A clinical-grade liquid biomarker detects neuroendocrine differentiation in prostate cancer. J Clin Invest. 2022 Nov 1;132(21): e161858. doi: 10.1172/JCI161858.PMID: 36317634.

Rodems TS, Heninger E, Stahlfeld CN, Gilsdorf CS, Carlson KN, Kircher MR, Singh A, Krueger TEG, Beebe DJ, Jarrard DF, McNeel DG, Haffner MC, Lang JM. Reversible epigenetic alterations regulate class I HLA loss in prostate cancer. Commun Biol. 2022 Sep 1;5(1):897. doi: 10.1038/s42003-022-03843-6. PubMed PMID: 36050516; PubMed Central PMCID: PMC9437063.

Bootsma M, McKay RR, Emamekhoo H, Bade RM, Schehr JL, Mannino MC, Singh A, Wolfe SK, Schultz ZD, Sperger J, Xie W, Signoretti S, Kyriakopoulos CE, Kosoff D, Abel EJ, Helzer KT, Rydzewski N, Bakhtiar H, Shi Y, Blitzer G, Bassetti M, Floberg J, Yu M, Sethakorn N, Sharifi M, Harari PM, Choueiri TK, Lang JM, Zhao SG. Longitudinal Molecular Profiling of Circulating Tumor Cells in Metastatic Renal Cell Carcinoma. J Clin Oncol. 2022 May 26; JCO2200219. doi: 10.1200/JCO.22.00219. [Epub ahead of print] PubMed PMID: 35617646.

Schehr JL, Sethakorn N, Schultz ZD, Hernandez CI, Bade RM, Eyzaguirre D, Singh A, Niles DJ, Henderson L, Warrick JW, Berry SM, Sundling KE, Beebe DJ, Leal TA, Lang JM. Analytical validation and initial clinical testing of quantitative microscopic evaluation for PD-L1 and HLA I expression on circulating tumor cells from patients with non-small cell lung cancer. Biomark Res. 2022 Apr 25;10(1):26. doi: 10.1186/s40364-022-003708. PubMed PMID: 35468853; PubMed Central PMCID: PMC9040226.

Heninger E, Kosoff D, Rodems TS, Sethakorn N, Singh A, Gungurthi H, Carlson KN, Yang B, Gilsdorf C, Pasch CA, Deming DA, Ellis L, Beebe DJ, Jarrard DF, Lang JM. Live cell molecular analysis of primary prostate cancer organoids identifies persistent androgen receptor signaling. Med Oncol. 2021 Sep 28;38(11):135. doi: 10.1007/s12032-021-01582-y. PubMed PMID: 34581895.

Daniel M, Knutson TP, Sperger JM, Li Y, Singh A, Stahlfeld CN, Passow C, Auch B, Lang JM, Dehm SM. AR gene rearrangement analysis in liquid biopsies reveals heterogeneity in lethal prostate cancer. Endocr Relat Cancer. 2021 Aug 11;28(9):645655. doi: 10.1530/ERC-21-0157. PubMed PMID: 34280123; PubMed Central PMCID: PMC8363559.

Sperger JM, Emamekhoo H, McKay RR, Stahlfeld CN, Singh A, Chen XE, Kwak L, Gilsdorf CS, Wolfe SK, Wei XX, Silver R, Zhang Z, Morris MJ, Bubley G, Feng FY, Scher HI, Rathkopf D, Dehm SM, Choueiri TK, Halabi S, Armstrong AJ, Wyatt AW, Taplin ME, Zhao SG, Lang JM. Prospective Evaluation of Clinical Outcomes Using a Multiplex Liquid Biopsy Targeting Diverse Resistance Mechanisms in Metastatic Prostate Cancer. J Clin Oncol. 2021 Jul 1: JCO2100169. doi: 10.1200/JCO.21.00169. [Epub ahead of print] PubMed PMID: 3419721

Bade RM, Schehr JL, Emamekhoo H, Gibbs BK, Rodems TS, Mannino MC, Desotelle JA, Heninger E, Stahlfeld CN, Sperger JM, Singh A, Wolfe SK, Niles DJ, Arafat W, Steinharter JA, Jason Abel E, Beebe DJ, Wei XX, McKay RR, Choueri TK, Lang JM. Development and initial clinical testing of a multiplexed circulating tumor cell assay in patients with clear cell renal cell carcinoma. Mol Oncol. 2021 Feb 19; doi: 10.1002/1878-0261.12931. [Epub ahead of print] PubMed PMID: 33604999.

Kyriakopoulos CE, Heath EI, Ferrari A, Sperger JM, Singh A, Perlman SB, Roth AR, Perk TG, Modelska K, Porcari A, Duggan W, Lang JM, Jeraj R, Liu G. Exploring Spatial Temporal Changes in 18F-Sodium Fluoride PET/CT and Circulating Tumor Cells in Metastatic Castration-Resistant Prostate Cancer Treated with Enzalutamide. J Clin Oncol. 2020 Nov 1;38(31):3662-3671. doi: 10.1200/JCO.20.00348. Epub 2020 Sep 8. PubMed PMID: 32897830

Pezzi HM, Guckenberger DJ, Schehr JL, Rothbauer J, Stahlfeld C, Singh A, Horn S, Schultz ZD, Bade RM, Sperger JM, Berry SM, Lang JM, Beebe DJ. Versatile exclusion-based sample preparation platform for integrated rare cell isolation and analyte extraction. Lab Chip. 2018 Nov 6;18(22):3446-3458. doi: 10.1039/c8lc00620b. PubMed PMID: 30334061; PubMed Central PMCID: PMC6402328.

Morris, RJ., Readio, N., Boland, K., Johnson, K., Lad, S., Singh, A., Singh, A., Holtorf, S., Skaar, S. Isolation of Mouse Epidermal Keratinocytes and Their In Vitro Clonogenic Culture. Journal of Visualized Experiments, (150), e58701, doi:10.3791/58701 (2019).

Park, H., Lad, S., Boland, K., Johnson, K., Readio,N., Jin, G., Asfaha, S., Patterson, KS., Singh, A., Yang, X., Londono, D., Singh, A., Trempus, T., Gordon, D., Wang, TC., and Morris, RJ. “Bone marrow derived epithelial cells and hair follicle stem cells are recruited to cutaneous neoplasms during tumor promotion of carcinogen-exposed skin”, Nature Communication, (9) 5293 (13 Dec 18), 2018.

Singh, A.,^Willems, E., Singh, A., Ong, IM., and Ajit K Verma. Ultraviolet radiation-induced differential microRNA expression in the skin of hairless SKH1 mice, a widely used mouse model for dermatology research. (*Corresponding author). Oncotarget. 2016 Oct 26. doi: 10.18632/oncotarget.12913.

Hafeez, BB., Meske, L., Singh, A., Singh, A., Zhong, W., Powers, P., John, M., Griep, AE., Verma, AK. Tissue-specific conditional PKCε knockout mice: a model to precisely reveal PKCε functional role in initiation, promotion and progression of cancer. Oncotarget, 2016. Vol 7, No. 22, 33067-33080.

Singh, A.,Singh, A., Willems, E., Hafeez, BB., Ong. IM., Mehta, SL., and Verma, A.K. Tumor necrosis factor-alpha and ultraviolet radiation which are linked to development of cutaneous SCC modulates differential epidermal microRNAs expression. Oncotarget, 2016, Vol. 7, No. 14, 17945-17956.

Singh, A., Singh, A., Sand, JM., Verma, A.K. Genetic deletion of TNFα in mice inhibits UVR-induced development of cutaneous squamous cell carcinomas via suppression of its own receptors and associated signals to epidermal cell survival. Carcinogenesis, 2016, Vol. 37, No.1, 72-80.

Singh, A., Singh, A., Sand, JM., Bauer, SJ., Hafeez, BB., Meske, L., and Verma, AK. Topically applied Hsp90 inhibitor 17AAG inhibits ultraviolet radiation-induced cutaneous squamous cell carcinomas. Journal of Investigative Dermatology. 2014. V-145, 1098-1107.

Singh, A., Singh, A., Sand, J.M., Heninger, E., Hafeez, B.B., and Verma, A. K. Protein Kinase Cε, which is linked to ultraviolet radiation-induced development of squamous cell carcinomas, stimulates rapid turnover of adult hair follicle stem cells. Journal of Skin Cancer. Vol 2013, Article ID 452425, 13 pages.

Singh, A., Park, H., Kangsamaksin, T., Singh, A., Readio, N., and. Morris, RJ. (2012). Keratinocyte Stem Cells and the Targets for Nonmelanoma Skin Cancer. Photochemistry and Photobiology. Vol 88, 1099-1110.